Artificial Insemination Frequently Asked Questions, Trenerth Equestrian
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Artificial Insemination - FAQs


How long does the dummy mare training take?

On average a stallion will be trained up in 2 weeks.


How long will my stallion be at your stud?

After he has done his training he can then use the walk in stallion service or he can stay at livery for the breeding season.


How long does my mare need to be at your stud?

We do provide a walk in AI service for your mare but this depends on you knowing the correct time of your mare's cycle.  Better results are usually achieved when the mare is left with us for the duration of her cycle so she can be scanned and given the AI at the correct time.  This would normally be a period of 3 to 4 weeks if her cycle is regular.


Can one sperm collection be used for more than one mare?

Yes depending on the quality of the sperm.


How long after collection can the sperm be used?

If semen is stored correctly up to 72 hours after collection.


What are your qualifications and who are you affiliated with?

I am a qualified AI Technicians approved with DEFRA and I am a member of A.I. of Equines Technicians Trade Association. 


My stallion/mare has a low fertility rate, how can AI help with this?

With AI we know the quality of the semen being used for your mare is good and with our expert veterinarian care we have had much success with problem mares in the past.


How do you perform artificial insemination?

First - collect the semen.  We do this by preparing an artificial vagina (AV) with hot water (temperature between 44˚C to 55˚C).  Each individual stallion can prefer different temperatures.

Next get rid of the gel and measure the semen volume.  You filter the semen to separate it from the gel and measure its volume using a clean (preferably new) container that has been pre-warmed to 37˚C.

Then extend the semen.  Semen extenders are usually milk based and protect the sperm against seminal plasma as well effects of ‘cold shock’. Semen in extender lasts longer and can be more easily examined to determine motility.

Evaluate the semen and look at the motility . Look under the microscope to determine the quality of the sperm and motility. Semen motility is measured as total and progressive. Progressive motility is a sperm that moves forward in a reasonably straight line and rotates its’ flat head as it swims.

Calculate the dose of semen.  Ideally about 500 X 106 progressively motile sperm (PMS) are needed to achieve consistent and maximum fertility. There are many stallions and many techniques that can be used to decrease this number but we strongly recommend starting with this number.

Breed the mare.  The raw semen is best added to extender early on after collection and the semen extender ratio used to calculate the raw semen constituent so as the mare is bred with the right number of PMS. To breed the mare, she is first carefully cleaned and rinsed. Soaps and cleaning agents tend be spermicidal. Then a gloved hand with a pipette is introduced into the vagina and the pipette passed gently through the cervix. The semen is then deposited into the uterine body.

Monitoring the mare whilst she is in season is vital to ensure that the insemination is timed correctly. When using chilled semen constant contact with the stallion owner is of great importance as this helps ensure that the semen arrives with the mare for insemination at the optimum time.